The Korea Institute of Toxicology (KIT) is a global toxicity research institute for the nation’s health and to make a more safe society. KIT is affiliated with National Research Council of Science and Technology (NST) which is under the Ministry of Science and ICT (MSIT) in South Korea. It was founded to contribute to the public health and welfare enhancement by safety assessments of chemical and biological materials by research developments of related technologies. KIT’s GLP system has been certified by Korean and international regulatory authorities based on OECD and US FDA GLP criteria. KIT was the first organization in Asia accredited by AAALAC International for humane laboratory animal treatment. Given that importance of alternative testing method gains momentum, the Department of Predictive Toxicology at KIT aims to develop advanced predictive toxicology technology based on in vitro and in silico approaches. KIT plays a leading role in developing alternative toxicology technology, and toxicity prediction AI model development is currently a key research project. Examples of AI models from KIT are drug-induced liver injury prediction model (http://toxstar.kitox.re.kr/), blood-brain barrier penetration prediction model (https://toxbbb.kitox.re.kr/), and nanotoxicity prediction models (OpenRiskNet services: https://openrisknet.org/e-infrastructure/services/ & NanoToxRadar: http://nanotoxradar.kitox.re.kr/). University of Science and Technology (UST) and KIT have founded a joint campus (called KIT campus in UST) to develop education program focused on human and environmental toxicology.